SIMPLE AND RAPID LIQUID CHROMATOGRAPHIC METHOD FOR REAL-TIME QUANTIFICATION OF NAPROXEN / ESOMEPRAZOLE MAGNESIUM COMBINATION TABLETS

Purpose: In this present investigation a simple, rapid and accurate reverse phase- liquid chromatographic method for the real- time quantification of naproxen and esomeprazole magnesium in tablet dosage form has been developed and validated. Materials and Methods: Isocratic elution mode with a mixture of phosphate buffer, pH 4.0 and acetonitrile in the ratio of 55:45 v/v was selected as the mobile phase with a universal C18 column (250x4.6mm, 5µm) by using waters alliance 2695 HPLC system with UV-detector. The retention time of naproxen and esomeprazole magnesium were found to be 2.229 min and 3.379 min, respectively at flow rate of 1.0ml/min with UV detector to evaluate at 306 nm. Results: A linear relation ship was found between peak area response and concentration in the range of 18.75 µg/mL- 112.5 µg/mL and 1µg/mL- 6 µg/mL for naproxen and esomeprazole magnesium. The % RSD values were found to be less than 2 for accuracy and precision studies. Conclusion: The method can be applied to quality control of pharmaceutical formulations containing naproxen and esomeprazole magnesium.