Alteraciones de la mucosa intestinal ante la presencia de Blastocystis sp. Estudio experimental

espanolCon el fin de estudiar los cambios citologicos que el Blastocystis ocasiona en el intestino delgado, se realizaron co-cultivos de mucosa intestinal de raton NMRI en presencia de la cepa BUEVG68-2008 de este microorganismo. La cepa fue recuperada mediante cultivo axenico de heces de pacientes con sintomatologia intestinal. La incubacion in vitro permitio observar la adhesion e internalizacion de celulas vacuolares de Blastocystis en las primeras 24h de cultivo, con la posterior alteracion del borde en cepillo intestinal a las 48h y destruccion del enterocito a las 72h, ocasionando la liberacion de abundantes detritos celulares y la presencia de macrofagos. Los hallazgos citologicos observados demuestran la capacidad de Blastocystis de alterar el epitelio intestinal. EnglishTo analyze the cytological changes occurring in the intestinal mucosa in the presence of Blastocystis, in vitro studies were performed using segments of the small intestine of NMRI mice incubated with a strain of Blastocystis sp. BUEVG68-2008, which was recovered by axenic culture from the stool of a child with intestinal symptoms. During the first 24 h of incubation, the initial interaction and internalization of vacuolated forms of Blastocystis sp. were observed, and between the next 48 and 72 h, alteration of the brush border and destruction of enterocytes were detected, causing the subsequent release of abundant cellular debris and presence of macrophages. The observed cytological findings demonstrate the capacity of Blastocystis to alter the intestinal epithelium.