Nonradioactive screening of glucokinase mutations in maturity onset diabetes of the young

DNA mutations were previously identified in the glucokinas gene in 56% of French families affected with maturity onset diabetes of the young (MODY), an early onset autosomal dominant from of non-insulin-dependent diabetes mellitus (NIDDM). Mutations were found on almost all exons using the common radioactive single-strand conformation polymorphism (SSCP) technique. In this paper, we describe a non-isotopic SSCP method using the Pharmacia Biotech PhastSystem for the routine screening of new mutations in diabetic patients or in offsprings of diabetic patients