Abstract 193: Analysis of Platelet Lysate Hydrogel and Fibrinogen Gel Intramuscular Injection on Neo-Angiogenesis in Ischemic Limbs

Introduction: We have recently characterized the in vitro benefits of the fibrin-rich pooled human platelet lysate (PL) in gel form on mesenchymal stem cells’ angiogenic activity. This study aimed to investigate the potential angiogenic activity of PL gel compared to a fibrin hydrogel (FG) in a murine model of hind limb ischemia (HLI). Methods: S129 male and female mice aged 20-weeks (n = 30) underwent right femoral artery ligation to induce HLI and were randomized to five groups: G1) Sham (n=5); G2) HLI-no treatment (n=5); G3) Saline (n=5; G4) PL (N=5); and G5) FG (n=5). The treatment limbs received PL, FG or saline by injection of 200 μL in the adductor muscle at day 0. Perfusion was quantitated with Laser Doppler Perfusion Imaging (LDPI) at 1-day post-procedurally and weekly for 28 days, and reported as ratios of the values in the ischemic limb (IL) to those in the contralateral nonischemic limb (NIL) for the adductor (thigh) and gastrocnemius (calf) areas. We then compared the change in the IL/NIL rat...