Cell line producing recombinant human erythropoietin and recombinant human erythropoietin produced by this cell

LINEAGE CELL ERYTHROPOIETIN HUMAN RECOMBINANT PRODUCTION AND HUMAN RECOMBINANT ERYTHROPOIETIN PRODUCED BY THIS cell . The gene coding for human erythropoietin (EPO) was obtained from human genomic DNA. The gene used does not include fragments flanking regions 5 'and 3' of the EPO gene which does not code for the protein. The gene was cloned into an expression plasmid for eukaryotic cells whose unique expression control elements the SV40 virus early promoter and its polyadenylation signal. The recombinant cells resulting from transfection with the genetic constructs used unexpectedly produces more than 50 mg of recombinant EPO per liter of culture medium per day.