Anti-inflammatory constituents from Perovskia atriplicifolia

AbstractContext: Perovskia atriplicifolia Benth (Labiantae) has long been used as a traditional herbal medicine for anti-inflammation in Pakistan; this prompted us to isolate anti-inflammatory compounds from this plant.Objective: The objective of this study was to isolate and characterize the anti-inflammatory principles from Perovskia atriplicifolia.Materials and methods: The CHCl3-soluble fraction of the methanol extract of the whole plant on column chromatography yielded compounds 1–6. The anti-inflammatory potential of the compounds 1–6 was evaluated by Leukotriene C4 (LTC4) Release Assay which was performed according to the established protocol. LTC4 in the supernatant of each well was measured using an ELISA kit (Cayman Chemical Co., Ann Arbor, MI).Results: The bioassay-guided phytochemical investigation of the CHCl3 soluble fraction of the methanol extract of Perovskia atriplicifolia furnished six compounds, abrotanone (1), abrotandiol (2), (+)-pinoresinol (3), (+)-syringaresinol (4), (+)-laricires...