Preparation of sophoridine liposomes by ammonium sulfate transmembrane gradient method.

Objective To investigate and optimize the formulation of sophoridine liposomes.Methods Sophoridine liposomes were prepared by ammonium sulfate transmembrane gradient method.On the basis of single factor experiments,the effects of influence factors,such as entrapment efficiency,drug loading,and comprehensive indexes,were investigated by using central composite design and response surface method.The influence factors included the concentration of drug and ammonium sulfate.The data were imitated using multi-linear equation and second-order polynomial equation.Results The latter was prior to the former considering from multiple correlation coefficients.Under the optimal conditions,the entrapment efficiency and drug loading of sophoridine liposomes were 51.81% and 5.39%.Conclusion The optimized preparation technique for sophoridine liposomes is stable and feasible.