Use of Premi®Test for the detection of sulphonamide residues in chicken eggs

The presence of sulphadimidine residues in eggs after a peroral administration of Sulfadimidin PG plv. sol. ad us, vet. (120 mg/hen/day) to laying hens was studied. Premi®Test, four-plate microbiological method, and HPLC were used for the detection of sulphadimidine residues. Positive findings of the Four-plate test (FPT) were confirmed by the results of Premi®Test. Using the FPT, the absence of sulphadimidine residues was confirmed 72 hours after the last sulphadimidine administration. The presence of sulphadimidine residues has been detected by Premi®Test within 8 days and by the FPT within 3 days after the last administration. As compared with the results of Premi®Test, the FPT has reported false-negative results for five days (kappa < 0.6). Conformity of results obtained by both Premi®Test and HPLC was confirmed in this study (kappa = 0.6).