Polymerase chain reaction (PCR) detection kit for discriminating *9A mutation locus in dihydropyrimidine dehydrogenase (DPYD) gene and method

The invention provides a real-time fluorescent PCR detection kit for discriminating a *9A mutation locus in a DPYD gene, which comprises specific primers, Eva Green fluorescent dye, PCR buffer solution, a deoxynucleotide triphosphate mixture and DNA polymerase, and is characterized in that the sequences of the specific primers are as follows: the sequence of the upstream primer is 5'-CCTGGCTTTAAATCCTCGAACA-3'; and the sequence of the downstream primer is 5'-AGGATTTCTTTTCCAATGTTTC-3'. The invention has the advantage that the kit can simply and conveniently discriminate the *9A mutation locus in the DPYD gene with high sensitivity and high specificity and can be added into a standard sample for quantitative detection and analysis.