Radioimmunoassay of secretin.

Several sensitive radioimmunoassay systems for secretin have been developed and reported1–6. The development of these assay systems depended upon the generation of a specific secretin antiserum and the iodination of secretin (natural porcine secretin, synthetic secretin or a tyrosyl derivative of secretin). This manuscript reports the results of studies conducted in our laboratory to validate a secretin radioimmunoassay.