The Influence of UVB Radiation on Proliferation and Youngs Modulus of Dermal Fibroblasts

The aim of this research is using different dose of UVB radiation to study the influence on dermal fibroblasts. These studies include cell viability analysis, proliferation rate and Youngs modulus measurement using Atomic force microscope (AFM). Results have exhibited that the cell viability was 100%, 89.46%, 73.76% and 70.75% when the exposed dose of UVB radiation on dermal fibroblasts was 0, 540, 1080 and 2160mJ/cm2. After 24 hours of incubation, dermal fibroblasts without exposure to UVB radiation increased cell growth to 183.5% but cells exposed to UVB radiation between 216 and 2160 mJ/cm2 reduced cell growth rate to 131% and 47.7%. Moreover, the Youngs modulus of dermal fibroblasts measured by AFM ranged from 24.12 to 9.40 kPa depending on the UVB exposed dose (0-2160 mJ/cm2). Therefore, UVB radiation showed a dose-dependent inhibitory effect on cell viability, proliferation rate and Youngs modulus.