Ki-1 (CD30) Positive anaplastic large cell lymphoma of T-cell phenotype developing in association with long-standing tuberculous pyothorax: Report of a case with detection of Epstein-Barr virus genome in the tumor cells

Abstract We report a case of CD30 positive anaplastic large cell lymphoma of T-cell phenotype developing in association with long-standing tuberculous pyothorax. Phenotypic analysis showed CD1a−, CD2+, CD3+, CD4+, CD5−, CD8−, CD10−, CD19−, CD20±, CD21−, CD25−, CD56−, T-cell receptor (TCR) alpha/beta antigens-, and HLA-DR+ phenotype. Neither rearrangement of TCR beta and gamma chain genes or of immunoglobulin heavy chain gene was detected in DNA extract from fresh materal. The lymphoma cells were also shown to express the latent membrane protein-1 and the Epstein-Barr virus (EBV)-encoded nuclear antigen-2 by immunohistochemistry and EBV-encoded small RNAs by in situ hybridization.