Inter- és intracelluláris jelátviteli mechanizmusok tér- és időbeli szerveződése gerinctelenek idegrendszerében = Spatial and temporal organization of inter- and intracellular signaling pathways in the nervous system of invertebrates

Inter- es intracellularis hirvivő rendszerek szerveződesenek kemiai-neuroanatomiai, hisztokemiai, neurokemiai es funkcionalis jellemzőit vizsgaltuk az osszehasonlito neurobiologia ket gastropoda modellallataban (Lymnaea stagnalis, Helix pomatia). i) Azonositottuk a vesztibularis erzekszervben, a statocystaban (Lymnaea) a szenzoros sejtek projekcios rendszeret, terbeli elrendeződesukett, kemiai hirvivőiket es szinaptikus bemeneteiket. ii) Azonositottuk a glutamaterg rendszer kozponti es periferias elemeit a felnőtt es fejlődő Lymnaea idegrendszereben, valamint a glutamat transzporterek (VGluT, EAAT) funkcionalis jellemzőit, molekularis szerkezetet es mRNS transzkripciojat. iii) Igazoltuk aminerg (szerotonin, dopamin) rendszerek meghatarozo szerepet a Lymnaea egyedfejlődesenek meneteben es felnőttkori magatartasformainak ereseben. iv) Kimutattuk a pofaizomzatban a szerotonerg innervacio es az izomrendszer parhuzamos ereset, tovabba azonositottuk a periferia (pofa- es talpizomzat) szerotonerg szabalyozasanak cAMPhez kapcsolt 5HT6-receptorat, illetve az embrionalis forgomozgas 5-HT1A receptorat. v) Azonositottuk es molekularis szinten differencialtuk a felnőtt es fejlődő kozponti idegrendszer szerveződeseben meghatarozo szerepet jatszo extracellularis matrix komponenseket. Eredmenyeink hozzajarulnak a neuronalis szignalizaciot, illetve magatartasformakat meghatarozo cellularis folyamatok, tovabba az idegi szabalyozas kemiai es molekularis esemenyeinek pontosabb megismeresehez. | Chemical-neuroanatomical, histochemical, neurochemical and functional organization of inter- and intracellular signaling systems were investigated in two model gastropod species of comparative neurobiology (Lymnaea stagnalis, Helix pomatia). i) Projection pattern, chemical heterogeneity and spatial distribution of the sensory hair cells of the vestibular organ (statocyst) of Lymnaea were identified. ii) Distribution and organization of the central and peripheral elements of the glutamatergic system in the adult and developing Lymanea were described, and the transporters (VGluT, EAAT) were cloned, in situ hybridized and functionally characterized. iii). The pivotal role of aminergic (serotonin, dopamine) systems in the embryogenesis and maturation of adult-like behaviors of Lymnaea was proven. iv) Timing and spatial organization of serotonergic innervation and buccal muscle development during Lymanea ontogenesis, as well as the serotonergic receptor cascade involved in feeding and locomotion (5-HT6-cAMP), and intracapsular rotation (5-HT1A-cAMP) was revealed. v). Extracellular matrix components playing a determining role in CNS organization and plastic events were identified and differentiated according to their molecular composition in adult and developing gastropods. The results contribute to a better understanding of neuronal signaling and regulatory processes underlying different behaviors, and to further analyze the chemical and molecular events of neuronal regulations.