Determination of Total Nitrogen in Tobacco and Tobacco Products by Automatic Kjeldahl Nitrogen Analyzer

The influences of amount of digesting agents,including CuSO4,K2SO4, and concentrated H2SO4,on the results in determining total nitrogen content in tobacco and tobacco product were investigated.The total nitrogen content in the samples was determined by an automatic steam distillation and titration device (automatic Kjeldahl nitrogen analyzer).The results showed that the average reco veries of nitrogen ranged from 97.90% to 103.85% with the coefficient of variati on below 2% when the sample was digested by 0.4 g CuSO4,6.0 g K2SO4,and 12 mL concentrated H2SO4.There is no significant difference between the test results of the mentioned method and YC/T161-2002 standard method.The proposed method i s suitable to the rapid analysis of total nitrogen in tobacco and tobacco produc ts.