The Synthesis of Radioiodinated Cobalt-bleomycin and its in vivo Distributions in Tumor-bearing Animals

This study is the addition of radioactive iodine on cobalt-bleomycin without changing its tumor-localizing properties.Cobalt-bleomycin demethy-A2 was synthesized by adding CoCl2 to bleomycin demethyl-A2 aqueous solution.On the other side, 3-125I-iodobenzylalcohol was prepared by replacing iodine of 3-iodobenzylalcohol with iodine-125I. And 3-125I-iodobenzyliodide was produced by adding P2I4 to 3-125I-iodobenzylalcohol in CH2Cl2.3-125I-iodobenzyl-cobalt-bleomycin was synthesized by coupling 3-125I-iodobenzyliodide to cobalt-bleomycin demethyl-A2.3-125I-iodobenzyl-cobalt-bleomycin was injected intravenously to tumor-bearing mice. In vivo distributions of this compound were measured from 60 minutes to 24 hours after injection.Accumulation of this compound in tumor tissue was much more than other normal organs except kidney and liver. This new labeled compound showed considerably strong affinity to malignant tumor.