From understanding to design

Part I. Basics and Manipulation of Light-Matter Interaction in Fluorescent Proteins One-Photon and Two-Photon Excitation of Fluorescent Proteins (Riccardo Nifosi) Primary Photophysical Processes in Chromoproteins (Steven Meech) Fluorescence Lifetime of Fluorescent Proteins (Gregor Jung) Synthetic Biology of Autofluorescent Proteins (Nediljko Budisa) Part II. Switching on the Molecular Level Vibrational spectroscopy: a tool to investigate the structure of the chromophore and its environment (Stefano Luin) Proton Travel in Green Fluorescent Protein (Volkhard Helms) Photoconversion of the Green Fluorescent Protein and Related Proteins (Jasper van Thor) Spectral Versatility of Fluorescent Proteins Observed on the Single Molecule Level (Christian Blum) Structure-Function Relationships in Fluorescent Marker Proteins of the Green Fluorescent Protein Family (Ulrich Nienhaus)