Carbohydrate Supplementation Before Operation Retains Intestinal Barrier Function and Lowers Bacterial Translocation in a Rat Model of Major Abdominal Surgery

Background: Overnight fasting of rats augments the susceptibility of the small intestine to ischemia–reperfusion damage. Feeding before surgery may improve injuries to distant organs that were induced by ischemia–reperfusion. The present study tested the hypothesis that one of the food constituents, namely carbohydrates, may be responsible for the protective effect of preoperative feeding on postoperative organ dysfunction. Methods: Male Wistar rats were fed ad libitum for 5 d and had either free access to water or free access to a carbohydrate drink and water. Then they were fasted for 16 h and access remained to either water or a carbohydrate drink and water. Following this, the arteria mesenterica superior was clamped for 60 min followed by 180 min of reperfusion. Subsequently, the intestinal permeability of stripped ileum was determined by measuring the mucosal to serosal flux in Ussing chambers. For assessment of bacterial content, organs were aseptically removed and assessed for bacterial content by...