Effects of NH_4NO_3 and Plant Growth Regulator on Callus Induction and Plant Regeneration from Anthurium andraeanum Lind.

Effects of NH4NO3 and plant growth regulator on callus induction and plant regeneration from Anthurium andraeanum Lind.were studied,with the leaves of Anthurium andreaenum cultivars Alabama and Sierra as explants.The results showed that,when the amount of NH4NO3 was reduced to 1/4 in the MS medium,the frequencies of callus induction of both cultivars were significantly increased by 285.9% and 458.5% respectively.The optimum culture medium for leaf callus induction was modified MS medium(1/4NH4NO3) supplemented with 0.4 mg/L TDZ and 1.0 mg/L 2,4-D.The optimum medium for adventitious bud induction and plantlet multiplication was 1/2MS+0.2 mg/L 6-BA+0.5 mg/L KT.Addition of 0.2 mg/L IBA was beneficial to plantlet growth.