TECHNIQUE FOR FRACTIONATION OF BACTERIA IN RUMEN MICROBIAL ECOSYSTEM. IV. ATTACHMENT OF RUMEN BACTERIA TO CELLULOSE POWDER AND ELUTION OF BACTERIA ATTACHED TO IT
1981
The influence of several conditions on the attachment of rumen bacteria to cellulose powder was examined. The attachment of bacteria to cellulose occurred shortly after the addition of the cellulose, and maximum attachment was attained after 5min further incubation at 38°. The amount of bacteria attached to cellulose was approximately proportional to the quantity of cellulose powder until 2% in weight per volume. Reaction temperature had a very profound effect on the attachment of bacteria to cellulose. The amount of bacteria attached to cellulose increased as the reaction temperature was raised. There existed bacteria capable of attaching to cellulose even at 4°, though the amount of the bacteria attached was small. The specific amylase, urease and protease activities of bacteria attached to cellulose were remarkably low, compared with those of non-attachable bacteria. Conversely, the specific β-glucosidase activitiy of bacteria attached to cellulose was significantly higher than that of non-attachable bacteria.A trial was made to elute bacteria attached to cellulose powder first with an anaerobic salt solution and subsequently with 0.1% aqueous solution of methylcellulose. The constitution of bacteria eluted from cellulose with the methylcellulose solution varied in detail with samples. The most predominant group among bacteria eluted with the methylcellulose solution from cellulose were gram-negative rods and they accounted for 60.1 to 80.1% of the total bacteria eluted. The results of the specific amylase, β-glucosidase and urease activities of bacteria eluted with the methylcellulose solution from cellulose were similar to those of bacteria attached to cellulose.
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