A comprehensive binding and neutralization analysis of plasma of HIV-1 subtype-C infected donors from India suggest MPER directed neutralization

Methods We tested 30 HIV-1 drug naive plasma samples for neutralization against a broad panel of subtype-A, B and C tier 1 and tier 2 viruses in a TZM-bl assay. Three broadly neutralizing plasma (bNP) samples (AIIMS206, AIIMS239 and AIIMS249) were tested for ELISA binding with a set of 211 consensus-C gp160 overlapping peptides (NIH AIDS Research and Reference Reagent Program). The competition and depletion experiments (for neutralization) were carried out with peptides corresponding to consensus-C V3 (35mer), IDR (19mer) and MPER (24mer).