HOW THE BIOGENIC AMINES AFFECT HAB ALGAE’S GROWTH: A PRELIMINARY EXPLORATION

Two species of dinoflagellates (Prorocentrum donghaiense, Alexandrium tamarense) and diatoms (Skeletonema costatum, Thalassiosira sp.) which are common in frequent-HAB area of East China Sea were selected in L16(45) orthogonal tests. Four biogenic amines, 2-phenylethylamine, putrescine, spermidine, and spermine, commonly detected in seawater during HAB time, were taken as test factors in four levels of 0, 5, 25, 100nmol/L. The nonlinear curve fitting with logistic growth model show that the influences varied in degree and trend when different biogenic amines acted on different HAB algae. Among them, 2-phenylethylamine was the most significant impact factor on the HAB algae’s growth. Spermidine affected A. tamarense and Thalassiosira sp. the most, while spermine had the biggest influence on P. donghaiense and S. costatum in three tested polyamines. Polyamines were considered as factors that affected the succession of HAB from S. costatum to P. donghaiense in East China Sea, 2010, of which spermine may be the dominating factor.