Isolation and identification of parvoviruses from raccoon dog.

Eight strains of virus were isolated from infected raccoon dogs from Daqing Prefecture in Heilongjiang Province by cultivation in Madin-Darby Canine Kidney(MDCK) cells.Then,the isolated viruses were identified by the neutralization test,hemagglutination test,physico chemical property test.The results showed that the isolated viruses could cause typical cytopathogenic effect(CPE) in MDCK cells at hour 72 after infection.The isolated virus could be neutralized with the positive sera with canine parvovirus(CPV) at titers from 1∶106 to 1∶107,could be agglutinated by the porcine erythrocytes and be inhibited by the positive sera with CPV.The isolates were insensitive to chloroform,aether,acid and temperature.A DNA fragment of 531 bp was amplified by PCR from the MDCK cells infected with isolated virus.The homology of VP2 gene sequence between the isolated viruses and EU310373 from GenBank was 99.6%.It was concluded from the results that the 8 isolated viruses from raccoon dog represent parvovirus.