Sequential Analysis of α-Glucooligosaccharides with α-(1→4) and α-(1→6) Linkages by Negative Ion Q-TOF MS/MS Spectrometry

Negative ion Q-TOF MS/MS spectra are shown to be very useful for sequential analysis of the glycosidic linkage in the α-gluco-oligosaccharides (DP 3-6) derived from an amylopectin molecule. The composition of the fragmentation ions generated from these compounds enabled us to distinguish two kinds of glycosidic linkage, α-(1→4) and α-(1→6), at same time to determine the glucose sequence from the reducing end of the oligosaccharide.