流行性造血器官坏死病毒(EHNV)PCR快速检测试剂盒的研制

Hematopoietic necrosis virus (EHNV) is a member of the genus Ranavirus (Iridoviridae), which can cause visceral necrosis in a variety of larvae and adult fish, and lead to the death of fish. In this study, we designed a pair of specific primers, using a method to isolate the DNA template rapidly and we optimized the PCR amplification conditions. These primers were then used to develop a rapid and sensitive PCR based kit for the detection of EHNV virus. Using this kit, the time for preparation of the DNA template was approximately 30 minutes, and accurate results could be obtained in approximately 4 hours. The amplification bands of the PCR product were specific, and the limit of detection was approximately 31 virions. This kit is fit for rapid diagnostic tests of early infection caused by EHNV, and water quality monitoring of fishery environments.