Up to Date In-vitro Artefacts in the Detection of Nanoparticles Toxicity: Short Review
2017
Nanoparticle toxicology is an emergent field that focuses on establishing the hazards of human exposure to
nanoparticles and their potential risk. Accurate assessments of nanoparticles risk involve the investigation of multiple
factors such as the nanoparticles parameters, the test system and the cell type. Some nanoparticles may interfere
with the toxicity detection assays or the enzymatic activity of the cell type. Thus, this lead to inaccurate obtained
data which could mislead researches. In this short review, we provided up to date assessment on the cause of
nanoparticles toxicity artefacts. Coating nanoparticle recently has been shown to hinder the interference with cell
viability assays; however, this was found to be cell type and concentration dependent. Therefore, researchers
suggest adding more washing steps to minimize the bound of nanoparticle with proteins or membranes. We suggest
that conducting an interference test for each nanoparticle prior toxicity assessment to avoid any flaws.
Keywords:
- Correction
- Source
- Cite
- Save
- Machine Reading By IdeaReader
22
References
3
Citations
NaN
KQI