Self-reinforcing injectable hydrogel with both high water content and mechanical strength for bone repair

Abstract The main challenge for constructing hydrogels serving as a temporary skeleton to support body load in bone tissue engineering is its poor mechanical strength. Covalent crosslinking is generally introduced in hydrogel to enhance its mechanical strength. However, this strategy always results in comitant reduction in the water content. In this study, a self-reinforcing injectable hydrogel based on noncovalent and Diels–Alder (DA) chemical dual crosslinking is developed to improve both its mechanical strength and water content. The noncovalent crosslinking is designed through the supramolecular interaction of cyclodextrin and adamantane, and the sol–gel transition of poly( N -isopropyl acrylamide) (PNIPAM), enabling hydrogel formation in situ after injection. DA chemical crosslinking occurs via furfurylamine grafted chondroitin sulfate (ChS-F) and maleimido-terminated poly(ethylene glycol) (PEG 2K –AMI), increasing the mechanical strength of hydrogel ( E  ∼ 25 MPa). In vivo bone repair tests were performed, and the results indicated that a cell- and factor-free, purely hydrogel-induced bone repair is achieved, demonstrating its potential in tissue engineering applications.