Construction of Recombinant Duck Plague Virus with VP1 Gene of Duck Hepatitis Virus Type I

In order to construct recombinant duck plague virus(DPV) containing VP1 gene of duck hepatitis virus type Ⅰ(DHV-Ⅰ),the VP1 gene of DHV-Ⅰ was amplified and inserted into EGFP expression cassette of TK gene-deleted transfer vector(pBlueSK-TK-EGFP) for DPV.The recombinant transfer vector(pBlueSK-TK-EGFP-VP1) was transfected into the DPV infected duck embryo fibroblasts(DEF) and the green fluorescence was still observed in cells after the third blind passage.A specific band(54 000) was detected in pBlueSK-TK-EGFP-VP1 transfected cells by Western-blot using positive serum of DHV-Ⅰ.These results showed that transfer vector pBlueSK-TK-EGFP-VP1 was successfully constructed and expressed in eukaryotic cells.