Prokaryotic expression and purification of PEDV N protein and analysis of B cell antigen epitope

The aim of this study was to clone and construct the porcine epidemic diarrhea virus (PEDV) N gene and to predict the epitope properties of its B cells The N gene of PEDV was amplified by PCR and cloned into the prokaryotic expression vector pET-28a(+) The positive clone was obtained by enzyme digestion, and the positive clone was expressed in the expression strain E coli BL21 (DE3) High purity expressed proteins were obtained by optimizing expression conditions and purification conditions The 3D structure of PEDV-N protein was established by bioinformatics homology modeling software Swiss-Pdb Viewer and the secondary structure, antigenicity, hydrophilicity and surface probability of PEDV-N protein were predicted according to bioinformatics software DNA-Star, and the antigen epitopes of its B cells were predicted by comprehensive analysis It is predicted that there were 13 B cell dominant epitopes in the amino acid sequence of PEDV-N protein The research results will further provide a theoretical basis for the application of PEDV-N protein expression products in vitro and the development of genetically engineered vaccines