Parâmetros de estresse oxidativo em camundongos submetidos a treinamento de Natação e overtraining

O objetivo deste trabalho foi comparar niveis de estresse oxidativo em camundongos da linhagem Balb-C submetidos a duas condicoes distintas de treinamento: treinamento moderado e progressivo de natacao (G-T) e inducao de overtraining (G-OVER). Foram investigados alguns marcadores de estresse oxidativo, tais como substâncias reativas ao acido tiobarbiturico (TBARS), proteinas carboniladas (PC) e grupamentos sulfidrila (GSH) no plasma e atividade das enzimas antioxidantes catalase (CAT) e glutationa redutase (GR) no hemolisado. Como marcadores de alteracao celular, quantificaram-se concentracoes plasmaticas das enzimas creatina quinase (CK), aspartato transferase (AST), creatinina (CRE) e ureia (UR). Como resultados importantes observamos que na situacao G-OVER houve queda significativa de CAT e GR (p<0,001) e CAT (p<0,05) em relacao a G-T, bem como aumentos significativos de TBARS, PC e CK (p<0,01) e AST, CRE e UR (p<0,001) em relacao a G-T. Concluiu-se que a alteracao do estado redox celular pode ser um importante objeto de estudo para deteccao de overtraining. ABSTRACT Oxidative stress parameters in mice submitted to swimming training and overtraining The aim of this study was to compare oxidative stress levels in mice (Balb-C) submitted to two different training conditions: moderate and progressive training (training group, T-G) and overtraining condition (G-OVER). We investigated some markers of oxidative stress, such as plasma thiobarbituric acid reactive substances (TBARS), protein carbonyls (PC) and sulfhydryl groups (GSH) and the antioxidant enzymes catalase (CAT) and glutathione reductase (GR) in hemolysate. As markers of cell damage was quantified plasma creatine kinase (CK) and aspartate transferase (AST) concentrations; were also observed patterns of physiological changes by quantifying plasma creatinine (CRE) and urea (UR). As the most important results, it was observed that in G-OVER a significant decrease of CAT and GR (p<0.001) compared to G-T, as well as significant increases in TBARS, PC and CK (p<0.01) and AST, CRE and UR (p<0.001) compared to G-T. We concluded that changes in cellular redox state can be an important object of study for overtraining detection.