The expression of microtubule-associated protein 1 light chain 3 、Beclin1 and osteopontin in aortic dissection mice's aortic tissue

Objective To explore the expression of microtubule-associated protein 1 light chain 3 、Beclin1 and osteopontinin aortic tissue of aortic dissection mice and to discuss the relevance.Methods (1) The establishment of aortic dissection model:3 weeks old FVB mice were randomly divided intoβ-aminopropionitrilemonofumarate (BAPN) with Angiotensin-Ⅱ (Ang-Ⅱ) group,BAPN only group and control group.The BAPN-Ang-Ⅱ group was intervened by BAPN orally at first 4 weeks and infused with Ang Ⅱ subcutaneously using a micro-osmotic pump for 48 h.The BAPN only group was intervened by BAPN orallyfor 4 weeks,and the control was given a placebo respectively.(2) Detecting the expression of microtubule-associated protein 1 light chain 3 、Beclin1 and osteopontin:RT-PCR and Immunohistochemistry staining was performed to detect the expression of LC3 B,Beclin1 and OPN in the aortic tissue of aortic dissectionmice.Results (1) the incidence of aortic dissection in the BAPN-Ang-Ⅱ is 86.7％ and the BAPN only group is lower than the collaborategroup.No aortic dissection was found in the control group.(2)It was identified that the autophagy related gene was much highly expressed in the AD mice's aortic tissue verse the normal one.According to immunohistochemistry staining,the autophagy related protein is mainly located in the medial vascular wall.Conclusion These data for the first time demonstrates that autophagy related gene and protein highly expressed in the AAD mouse's aortic tissue and it will provide us an available method and fundamental for the further studying in the influences of autophagy to AAD. Key words: Aortic dissection ;  Aorta ;  Autophagy ;  Vascular smooth muscle cells ;  Animal experiment ; Microtubule-associated protein 1 light chain 3 ;  Beclin1 ;  Osteopontin