Attraction of the grapevine moth to host and non-host plant parts in the wind tunnel: effects of plant phenology, sex, and mating status

In the European grapevine moth, Lobesia botrana (Denis and Schiffermuller) (Lepidoptera: Tortricidae), it has recently been shown that volatiles emitted from the main host plant, grapevine, are attractive to adult females. Here, using wind tunnel experiments, we tested the attractiveness of various grapevine, Vitis vinifera L. (Vitaceae), plant parts at different phenological stages, including ripe berries infested with the pathogenic fungus Botrytis cinerea (Persoon: Fries) (Sclerotiniaceae) to laboratory-reared virgin and mated male and female moths. We also tested the attractiveness of the non-host plant, tansy [Tanacetum vulgare (L.) (Asteraceae)], as flowers and flower volatiles were previously shown to be attractive to L. botrana females in the field. Mated female moths were the only adults to exhibit upwind orientation to grape plant parts in the flight tunnel. The most attractive parts of the grapevine plants were leaves, flower buds, and ripe berries. No attraction was observed to flowers. This corresponds to the phenological stages available when females of the 2-3 generations during a year are present in the field. No attraction was observed to leaves and flowers of tansy.