Genotyping of Circulating Tumor DNA Reveals the Clinically-actionable Mutation Landscape of Advanced Colorectal Cancer

Circulating tumor DNA (ctDNA) enables genomic profiling of colorectal cancer (CRC). We investigated therapeutic targets by performing ctDNA panel-captured sequencing of 152 blood samples from advanced-stage patients, from which somatic mutations and potentially actionable targets were evaluated. An additional 11 matched tissue samples were retrospectively obtained to verify target validity. The mutation frequencies of 1,127 collective genetic variants identified in our study strongly correlated with those of multiple public databases (Pearson R2=0.92, p