Validation of standards suitable for genome size estimation of fungi

Abstract Genome size information is fundamental to genome sequencing and may also uncover genomic aspects of evolution. Flow Cytometry, the preferred method for genome size estimation, requires suitable standards. Here we validate Inonotus hispidus , Colletotrichum acutatum and Cenococcum geophilum (41, 68 and 203 Mbp), as standards for fungal genome size estimation.