Anti-Inflammatory, Antinociceptive and CNS Depressant Activities of the Methanolic Extract of Phyllanthus reticulatus Leaves

Objectives- The study was carried out to assess the anti-inflammatory, analgesic and CNS depressant activity of the methanolic extract of leaves of Phyllanthus reticulatus (MEPR). Methodology- Anti- inflammatory effects of MEPR was done by carrageenan induced inflammatory method at the dose of 100 and 200 mg/kg b.wt.,i.p. A significant (p<0.05) inhibition of paw oedema at 2, 3 and 4 hours as compared to reference drug diclofenac were observed. The acetic acid-induced writhing model and formalin test method determined the analgesic activity of the MEPR in mice. The extract showed 64.628% inhibition whereas 66.446% inhibition was observed for diclofenac sodium against pain in the late phase of the formalin induced test. The CNS depressant activity was evaluated by observing the reduction of locomotor and exploratory activities in the open field and hole cross tests at dose of 250 and 500 mg/kg body weight. The results of the statistical analysis showed that the plant extract have significant (p<.05) dose dependent CNS depressant. In conclusion this study recommend that methanolic extract of leaves of Phyllanthus reticulatus has anti-inflammatory, analgesic and CNS depressant properties.