Low-Coherence Shearing Interferometry With Constant Off-Axis Angle

We present a wide-field interferometric imaging module for biomedical and metrological measurements, employing shearing interferometry with constant off-axis angle (SICA) that can work, for the first time, with a low-coherence light source. In the SICA module, the off-axis angle can be fully controlled without a direct relation with the shearing distance between the interfering beams. In contrast to our previous SICA module, here we use a low-coherence illumination source, providing quantitative phase profiles with significantly lower spatial coherent noise. Although a low-coherence source is used, we obtain off-axis interference on the entire camera sensor, where the optical path difference between the two beams is compensated by using a glass window positioned in the confocal plane. This highly stable common-path low-coherence single-shot interferometric module can be used as an add-on unit to a conventional bright-field microscope illuminated by a low-coherence source. We demonstrate the advantages of using the module by quantitative phase imaging of a polymer bead, fluctuations in human white blood cell, and dynamic human sperm cells.