Cloning and Expression Analysis of hsf from Lilium longiforum

A all-length heat shock transcription factor(HSF) gene with 350aa ORF,belonging to HSFA,was cloned from Lilium longiforum ‘white heaven’ by RACE(Rapid Amplification of cDNA Ends).Cluster analysis showed that this gene was highly homologous to known HSFA2 from other organisms and one novel member of HSFA2.Furthermore,alignment with HSFA2 from tomato and Arabidopsis indicated there was highly conserved DNA binding domain in this gene,harboring other characterized domains and regulatory elements.RT-PCR showed that expression of this gene could be detected in roots and bulbs treated by 37 ℃ for 1 hour and be detected in leaves during 0.5–12 hours at 37 ℃.