DETECTION OF NAD(P)H: QUINONE OXIDOREDUCTASE 609C?T POLYMORPHISM IN BLOOD AND ARCHIVAL HUMAN TISSUES USING A SIMPLE PCR METHOD

NAD(P)H: quinone oxidoreductase (NQO1) plays an important role in detoxification of numerous endogenous and foreign compounds. This gene has a single nucleotide polymorphism at site of codon 187 (CCT TCT). Recently, it has been demonstrated that individuals with T allele may exhibit resistance to quinone based anticancer drugs such as mitomycin C. In the present study, a simple and feasible method was developed for detection of NQO1 genotype. In this modified procedure, dimethylsulfoxide (DMSO) and Triton X-100 were eliminated, also, PCR cycling conditions were modified to improve the PCR products from blood and formalin-fixed, paraffin-embedded tissues. PCR-RFLP and DNA sequencing analysis carried out on a limited number of blood and archival samples. It is suggested that this procedure convenient for NQO1 genotyping.