To Investigate the Effect of Using Ethanol Containing Wipes in Collecting Blood for the Measurement of Alcohol Concentration
2021
This study was aimed to establish whether the skin
preparation using ethanol-containing skin antiseptics causes ethanol
contamination through blood collection. Venous blood was collected from 40 healthy
volunteers according to the national guidelines for blood sampling, with four
sequential procedures as follows: 1) collecting blood immediately (within 5
seconds) after cleaning the skin with an individually packaged type of
ethanol-containing wipe, 2) collecting blood 1 minute after cleaning the skin
with an individually packaged type of ethanol-containing wipe, 3) collecting
immediately (within 5 seconds) after cleaning the skin with a traditional
cleaning method (thoroughly ethanol-impregnated wipe, and 4) collecting 1
minute after cleaning the skin with a traditional cleaning method. Each
sequential procedure was performed with and
without the ethanol-containing wipe used for skin cleaning on the
puncture site on their right and left arms at the time the needle was
withdrawn, respectively. The collected specimens were subjected to the
determination of ethanol by using headspace gas chromatography-mass
spectrometry. In every 80 blood specimens obtained from 40 participants,
ethanol was undetectable (<0.001 mg/mL). This study demonstrates that
disinfection using ethanol-containing skin antiseptics is unlikely to cause
ethanol contamination through blood collection regardless of skin preparation
technique according to the guidelines for blood sampling. This may have
implications in forensic science.
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