Establishment of Implanted Tumor Models of Human Bile Duct Carcinoma in the Nude Mice.

Objective:To establish the implanted tumor models of human bile duct carcinoma in the nude mice.Methods:Surgical specimens of patients with bile duct carcinoma were implanted into the subcutaneous layer and liver of nude mice.Growth lines of implanted tumors were drawn and its morphological and biological characteristics observed.Results:A well differentiated gelatinous adenocarcinoma model and a moderately differentiated papillary adenocarcinoma model of human bile duct carcinomas in nude mice were established and named HBDCM1-ZSH and HBDCM2-ZSH fespectively.The growth rate of subcutaneous implantation was40%;implanted tumors of model1and model2tumors exhibited97.7%and100%implantability in the nude mice,their latent periods were26d and21d respectively and exhibited the characteristics of the original tumors both morphologically and biologically.Conclusion:Models HBDCM1-ZSH and HBDCM2-ZSH resemble to their original human bile duct carcinoma types and provide good animal models for bile duct carcinoma research.