Modified human c hepatitis virus genome rna having autonomous replication ability

The invention, of two or more genomic RNA of hepatitis C virus, the 5 'untranslated region, core protein coding sequence, E1 protein coding sequence, p7 protein coding sequence, E2 protein coding sequence, NS2 protein coding sequence, NS3 protein coding sequence, NS4A protein coding sequence, NS4B protein coding sequence, NS5A protein coding sequence, consists NS5B protein coding sequence, and 3 'nucleotide sequence including untranslated regions, and having autonomous replication ability, modified hepatitis C virus to provide a genomic RNA. In particular, the NS3 of the hepatitis C virus genome RNA NS4, NS5A, an RNA sequence portion encoding the NS5B protein, NS3 of JFH1 strain shown in SEQ ID NO: 1, NS4, NS5A, and RNA subsequences encoding NS5B protein by replacing, it won autonomous replication ability, to hepatitis C virus genomic RNA that has been modified.