Fatty acid in lipopolysaccharides of Salmonella species grown at low temperature: identification and position

Salmonella minnesota R 595 (Re) and other Salmonella strains incorporate cis-Δ9-16:1 (palmitoleic acid) at the expense of mainly dodecanoic acid into the lipid-A portion of lipopolysaccharides, when the cells are grown at low temperature (12°C). It has recently been shown, that in S. minnesota R 595 grown at 37°C dodecanoic acid is linked to the 3-hydroxyl group of an amide-bound 3-hydroxytetradecanoic acid. The present data suggest, that cis-Δ9-16:1 occupies the same position in lipid A (12°C).