Construction of a Glucose Biosensor by Immobilizing Glucose Oxidase within a Poly(o- phenylenediamine) Covered Screen-printed Electrode

The glucose biosensors were prepared by the electropolymerization of the non-conductive polymer, Poly(o-phenylenediamine), onto a planar screen-printed electrode. A fabrication procedure was performed to decrease the waste of expensive enzyme. The amperometric glucose response was measured by the potensiostating of the prepared glucose biosensors at a potential of 0.3 V with ferrocene as mediator. Results show that the obtained biosensors have a linear range up to 25 mM glucose, fast response time (100s) and high sensitivities (16.6 nA/mM). Also, the effects of applied potential and sweeping number of Cyclic voltammograms for electropolymerization were systematically investigated and optimal values were recorded.