PHCOG MAG.: Research Article In vitro effects of extract of Senna alata (Ceasalpiniaceae) on the polyamines produced by Leukaemia cells (L1210)

The present study reports the effects of S. alata (Ceasalpiniaceae) extract on the metabolism of polyamines resulting from the proliferation of leukaemia cells (L1210). The results established that the inhibition of cell proliferation was significantly increased with the concentration of extract from 28 to 32.80 % after 72 h. The percentage of cells viability changed significantly from 9.72 to 80 % when cells are treated with extract alone, in combination with DFMO or putrescine. The levels of the intracellular yield of putrescine, spermidine and spermine were also reduced by the extract compared to the control. The DFMO-extract complex enhanced the inhibition of the production polyamines up to 95 %. In opposite, the complex S. alata - putrescine complex stimulated significantly its biosynthesis of polyamines. A significant reduction of the level of protein after 72 h of treatment was observed. This result corroborated with the reduction of polyamines resulting from inhibition cell