All-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins

Daniel Hochbaum,Yongxin Zhao,Samouil L. Farhi,Nathan C. Klapoetke,Christopher A. Werley,Vikrant Kapoor,Peng Zou,Joel M. Kralj,Dougal Maclaurin,Niklas Smedemark-Margulies,Jessica L. Saulnier,Gabriella L. Boulting,Christoph Straub,Yong Ku Cho,Michael Melkonian,Gane Ka-Shu Wong,D. Jed Harrison,Venkatesh N. Murthy,Bernardo L. Sabatini,Edward S. Boyden,Robert E. Campbell,Adam E. Cohen

All-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins

2014

A combination of a sensitive blue light–gated channelrhodopsin actuator and red-shifted Arch-based voltage sensors allows all-optical electrophysiology without cross-talk in cultured neurons or brain slices.

Keywords:

Channelrhodopsin
Biophysics
Directed Molecular Evolution
Rhodopsin
Electrophysiology
Molecular biology
Fluorescence-lifetime imaging microscopy
Neurotransmission
Biology
Optogenetics
all optical

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