Genetic Diversity of Bacteriophage Communities in Napahai Wetland

Many results have showed that the great diversity of bacteriophages in various aquatic environments, including marine water, freshwater and paddy water, but few studies was about the genetic diversity of bacteriophages in the wetland. To better understand the genetic diversity of bacteriophages communities of Napahai wetland, we selected viral capsid protein gene (g23) as a gene marker to reveal genetic diversity of bacteriophage in Napahai wetland in Yunnan province of China. The g23 gene fragment of bacteriophage was amplified with the primers MZIA1Bis and MIZA6. In this study, 21 different clones fragments in g23 gene sequences were found. Phylogenetic analysis demonstrated that some g23 genotypes were closely related to the T4-like phages, the others clustered two groups were unknown sequences which had far evolutionary distances with the currently known phages sequences. So the unknown sequences may represent the new particular bacteriophage communities. Keywords-T4-type phage; Napahai wetland; g23 gene; Genetic diversity.