Screen of a membrane protein molecule from Vero cells capable of binding Japanese encephalitis virus(JEV)

AIM:Identification of membrane protein molecules Vero cells that binding JEV.METHODS:Membrane protein extract was subject to co-immunoprecipitation (Co-IP) with JEV,and identified by mass (MS) spectrometry.The binding between specific extract protein and JEV was measured by MS,flow cytometry (FCM) and immunofluorescence assay (IFA).RESULTS:Heat shock protein 90 β (HSP90 β) was identified by Co-IP and MS,and its binding JEV activity was confirmed by FCM and IFA.CONCLUSION:HSP 90 β from Vero cells membrane could bind JEV.