The role of JAK/STAT signaling pathway and TNF-α crosstalk in human colorectal cancer

Abstract Colorectal cancer (CRC) constitutes a significant portion of mortality and morbidity due to cancer around the world. The present study was designed to evaluate the possible effects of the JAK/STAT, TNF-α, and NFκB pathways, and the caspase-3 apoptotic marker in human colorectal tissue. Data from tumor tissue and adjacent normal colon tissue (n:12) were obtained. Given the biological role of the JAK/STAT signaling pathway in human colorectal tissue, we evaluated interactions with TNFα, NFκB, and caspase-3. We measured the mRNA levels of STAT1, TNF-α, TNF-α R1A, NFκB, and caspase-3 by RT-PCR. The STAT1, TNF-α, TNF-α R1A, and NFκB mRNA levels were increased in colorectal cancer tissue compared to adjacent normal colon tissue ( P P P P