An experimental study of hyperthermochemotherapy without oxygenation by isolated liver perfusion

: The new method of hyperthermochemotherapy by the isolated liver perfusion technique without oxygenation is developed using beagle dogs. In this study, we investigated the influence of this method on the liver and other organs. The isolated liver perfusion was performed at the rate of 75-100 ml/min through the portal vein for 30 min under the V-V bypass. Experimental groups were divided as following; Croup I (n = 5): The perfusate was lactate Ringer's solution without oxygenation and kept at 42 degrees C. Group II (n = 5), Mitomycin C (10 micrograms/ml) was added to the perfusate used in Group I. During the perfusion the liver temperature was kept at 41 degrees C. PH of the liver and AKBR returned quickly to preperfusion level after liver perfusion. In the both groups, GOT, GPT, ALP were elevated, and returned to normal range within 14 days, although GPT was significantly high until the 5th day in Group II compared with Group I. In the values of amylase, BUN and Cr., no remarkable changes were observed, and any histologic damage of the liver and other organs was not found on day 14. In conclusion, this new hyperthermochemotherapy system could be a simple and safe method, and should be applicable for clinical cases.