The display substrate and a method of preparing a display device

The present disclosure provides a display substrate types and preparation methods, a display device, the production method comprising: providing a base substrate; a pixel define layer formed on the base substrate; the pixel defining layer to an oxidation treatment, wherein said a pixel defining layer remote from the substrate side of the substrate is partially oxidized, thereby making the pixel defining layer comprises a layer adjacent to the body and away from the base substrate of the oxide layer of the base substrate; definition of the pixel curing layer forming process, and the pixel defining layer is patterned to form a pixel defining layer process pattern. Interface Issues pixel defining layer is patterned by the deposition process and patterning process times, simplifying the preparation process and reducing the manufacturing cost, and is formed inside the pixel define layer pattern is not present, can improve the stability of the pixel define layer pattern structure.