Toxicidad aguda de un extracto acuoso de Boldoa purpurascens Cav. en el modelo de sube y baja en ratas

Fundamentos: Boldoa purpurascens Cav (nitro blanco) ha sido utilizada tradicionalmente como diuretica en Cuba, sin embargo, no cuenta con los estudios toxicologicos preclinicos establecidos. Objetivos: determinar la toxicidad aguda de un extracto acuoso liofilizado de hojas secas de B. purpurascens. Metodos: se realizo un estudio preclinico mediante el metodo de sube y baja (UDP), el grupo experimental estuvo conformado por 5 ratas hembras de la linea Sprague Dawley. Se administro una dosis limite diaria de 2 000 mg/kg. Se realizaron observaciones sistematicas durante el transcurso del experimento y el dia 14 posadministracion se procedio a la eutanasia de los animales. Resultados: el peso corporal se comporto acorde a la curva de crecimiento de la especie, sin evidencia de signos toxicos ni muerte de ningun animal. El estudio anatomopatologico macroscopico de los organos no mostro alteraciones. Conclusiones: la dosis toxica del extracto estudiado es superior a 2 000 mg/kg y clasifica como no toxico. Foundations: Boldoa purpurascens Cav (nitro blanco) has been traditionally used as a diuretic in Cuba; however, the established preclinical toxicological studies have not been conducted yet. Objectives: to determine acute toxicity in a freeze-dried aqueous extract form dry leaves of B. Purpurascens. Methods: a preclinical study was undertaken by the up and down procedure. The experimental group was composed of 5 Sprague Dawley female rats. A daily limited dose of 2 000 mg/kg was administered. Systematic observations were made during the experiment and on the 14th postadministration day, the euthanasia of the animals was performed. Results: the body weight behaved according to the growth curve of the species without evidence of toxic signs or death of any animal. The macroscopic anatomopathological study of the organs showed no alterations. Conclusions: the toxic dose of the studied extract is higher than 2 000 mg/kg and classifies as non-toxic.